2B63

Complete RNA Polymerase II-RNA inhibitor complex

2B63 の概要

• エントリーDOI: 10.2210/pdb2b63/pdb
• 分子名称: 31-MER, DNA-directed RNA polymerase II subunit 9, DNA-directed RNA polymerases I/II/III subunit 10, ... (15 entities in total)
• 機能のキーワード: rna polymerase ii, rna, aptamer, protein-rna complex, inhibitor, transferase-rna complex, transferase/rna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P38902 P08518 P16370 P20433 P20434 P34087 P20436 P27999; Nucleus, nucleolus: P22139 P40422; Cytoplasm: P20435
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 525029.34

構造登録者

Kettenberger, H.,Eisenfuehr, A.,Brueckner, F.,Theis, M.,Famulok, M.,Cramer, P. (登録日: 2005-09-30, 公開日: 2005-12-06, 最終更新日: 2024-11-20)

主引用文献

Kettenberger, H.,Eisenfuehr, A.,Brueckner, F.,Theis, M.,Famulok, M.,Cramer, P.
Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs
Nat.Struct.Mol.Biol., 13:44-48, 2006

PubMed Abstract: The noncoding RNA B2 and the RNA aptamer FC bind RNA polymerase (Pol) II and inhibit messenger RNA transcription initiation, but not elongation. We report the crystal structure of FC(*), the central part of FC RNA, bound to Pol II. FC(*) RNA forms a double stem-loop structure in the Pol II active center cleft. B2 RNA may bind similarly, as it competes with FC(*) RNA for Pol II interaction. Both RNA inhibitors apparently prevent the downstream DNA duplex and the template single strand from entering the cleft after DNA melting and thus interfere with open-complex formation. Elongation is not inhibited, as nucleic acids prebound in the cleft would exclude the RNA inhibitors. The structure also indicates that A-form RNA could interact with Pol II similarly to a B-form DNA promoter, as suggested for the bacterial transcription inhibitor 6S RNA.

PubMed: 16341226
DOI: 10.1038/nsmb1032

実験手法

X-RAY DIFFRACTION (3.8 Å)