2B5A

C.BclI, Control Element of the BclI Restriction-Modification System

2B5A の概要

• エントリーDOI: 10.2210/pdb2b5a/pdb
• 分子名称: C.BclI, ACETIC ACID (3 entities in total)
• 機能のキーワード: helix-turn-helix motif, gene regulation
• 由来する生物種: Bacillus caldolyticus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 35845.43

構造登録者

Sawaya, M.R.,Zhu, Z.,Mersha, F.,Chan, S.H.,Dabur, R.,Xu, S.Y.,Balendiran, G.K. (登録日: 2005-09-28, 公開日: 2006-01-03, 最終更新日: 2024-02-14)

主引用文献

Sawaya, M.R.,Zhu, Z.,Mersha, F.,Chan, S.H.,Dabur, R.,Xu, S.Y.,Balendiran, G.K.
Crystal Structure of the Restriction-Modification System Control Element C.BclI and Mapping of Its Binding Site.
Structure, 13:1837-1847, 2005

PubMed Abstract: Protection from DNA invasion is afforded by restriction-modification systems in many bacteria. The efficiency of protection depends crucially on the relative expression levels of restriction versus methytransferase genes. This regulation is provided by a controller protein, named C protein. Studies of the Bcll system in E. coli suggest that C.Bcll functions as a negative regulator for M.Bcll expression, implying that it plays a role in defense against foreign DNA during virus infection. C.Bcll binds (Kd = 14.3 nM) to a 2-fold symmetric C box DNA sequence that overlaps with the putative -35 promoter region upstream of the bcllM and bcllC genes. The C.Bcll fold comprises five alpha helices: two helices form a helix-turn-helix motif, and the remaining three helices form the extensive dimer interface. The C.Bcll-DNA model proposed suggests that DNA bending might play an important role in gene regulation, and that Glu27 and Asp31 in C.Bcll might function critically in the regulation.

PubMed: 16338412
DOI: 10.1016/j.str.2005.08.017

実験手法

X-RAY DIFFRACTION (1.543 Å)