2B4Z

Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.

2B4Z の概要

• エントリーDOI: 10.2210/pdb2b4z/pdb
• 分子名称: Cytochrome c, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: ferric form, low ionic strength, electron transport
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Mitochondrion matrix: P62894
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12211.88

構造登録者

Mirkin, N.,Jakoncic, J.,Stojanoff, V.,Moreno, A. (登録日: 2005-09-27, 公開日: 2005-10-11, 最終更新日: 2023-08-23)

主引用文献

Mirkin, N.,Jaconcic, J.,Stojanoff, V.,Moreno, A.
High resolution X-ray crystallographic structure of bovine heart cytochrome c and its application to the design of an electron transfer biosensor.
Proteins, 70:83-92, 2008

PubMed Abstract: Cytochrome c is one of the most studied proteins probably due to its electron-transfer properties in aerobic and anaerobic respiration. Particularly, cytochrome c from bovine heart is a small protein, M(r) 12,230 Da, globular (hydrodynamic diameter of 3.4 nm), soluble in different buffer solutions, and commercially available. Despite being a quite well-studied protein and relatively easy to manipulate from the biochemical and electrochemical viewpoint, its 3D structure has never been published. In this work, the purification, crystallization and 3D structure of one of the cytochrome c isoforms is presented to 1.5 A resolution. It is also shown how the presence of isoforms made both the purification and crystallization steps difficult. Finally, a new approach for protein electrocrystallization and design of biosensors is presented.

PubMed: 17634981
DOI: 10.1002/prot.21452

実験手法

X-RAY DIFFRACTION (1.5 Å)