2B4E

Crystal Structure of Murine Coronin-1: monoclinic form

2B4E の概要

• エントリーDOI: 10.2210/pdb2b4e/pdb
• 関連するPDBエントリー: 2AQ5
• 分子名称: Coronin-1A (2 entities in total)
• 機能のキーワード: wd40 repeat, 7-bladed beta-propeller, structural protein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm, cytoskeleton: O89053
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44360.68

構造登録者

Appleton, B.A.,Wu, P.,Wiesmann, C. (登録日: 2005-09-23, 公開日: 2005-12-06, 最終更新日: 2023-08-23)

主引用文献

Appleton, B.A.,Wu, P.,Wiesmann, C.
The crystal structure of murine coronin-1: a regulator of actin cytoskeletal dynamics in lymphocytes
Structure, 14:87-96, 2006

PubMed Abstract: Mammalian coronin-1 is preferentially expressed in hematopoietic cells and plays a poorly understood role in the dynamic reorganization of the actin cytoskeleton. Sequence analysis of coronin-1 revealed five WD40 repeats that were predicted to form a beta propeller. They are followed by a 130 residue extension and a 30 residue leucine zipper domain that is responsible for multimerization of the protein. Here, we present the crystal structure of murine coronin-1 without the leucine zipper at 1.75 A resolution. Coronin-1 forms a seven-bladed beta propeller composed of the five predicted WD40 repeats and two additional blades that lack any homology to the canonical WD40 motif. The C-terminal extension adopts an extended conformation, packs tightly against the bottom surface of the propeller, and is likely to be required for the structural stability of the propeller. Analysis of charged and conserved surface residues delineate possible binding sites for F-actin on the beta propeller.

PubMed: 16407068
DOI: 10.1016/j.str.2005.09.013

実験手法

X-RAY DIFFRACTION (2.3 Å)