2B2D

RNA stemloop operator from bacteriophage QBETA complexed with an N87S,E89K mutant MS2 capsid

2B2D の概要

• エントリーDOI: 10.2210/pdb2b2d/pdb
• 関連するPDBエントリー: 1AQ3 1AQ4 1BMS 1DZS 1E6T 2B2E 2B2G 2BNY
• 分子名称: 5'-R(*AP*UP*GP*CP*AP*UP*GP*UP*CP*UP*AP*AP*GP*AP*CP*AP*GP*CP*AP*U)-3', Coat protein (3 entities in total)
• 機能のキーワード: capsid, complex (capsid protein - rna hairpin) hairpin, levivirus, virus/viral, protein/rna, virus-viral protein-rna complex, virus/viral protein/rna
• 由来する生物種: Enterobacterio phage MS2
• 細胞内の位置: Virion (Potential): P03612
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 53918.24

構造登録者

Horn, W.T.,Tars, K.,Grahn, E.,Helgstrand, C.,Baron, A.J.,Lago, H.,Adams, C.J.,Peabody, D.S.,Phillips, S.E.V.,Stonehouse, N.J.,Liljas, L.,Stockley, P.G. (登録日: 2005-09-19, 公開日: 2006-05-09, 最終更新日: 2023-10-25)

主引用文献

Horn, W.T.,Tars, K.,Grahn, E.,Helgstrand, C.,Baron, A.J.,Lago, H.,Adams, C.J.,Peabody, D.S.,Phillips, S.E.V.,Stonehouse, N.J.,Liljas, L.,Stockley, P.G.
Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2
Structure, 14:487-495, 2006

PubMed Abstract: Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.

PubMed: 16531233
DOI: 10.1016/j.str.2005.12.006

実験手法

X-RAY DIFFRACTION (2.9 Å)