2B1E

The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif

2B1E の概要

• エントリーDOI: 10.2210/pdb2b1e/pdb
• 関連するPDBエントリー: 2D2S
• 分子名称: Exocyst complex component EXO70 (2 entities in total)
• 機能のキーワード: tethering complex, exocyst, endocytosis-exocytosis complex, endocytosis/exocytosis
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Bud: P19658
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 64803.81

構造登録者

Dong, G.,Hutagalung, A.H.,Fu, C.,Novick, P.,Reinisch, K.M. (登録日: 2005-09-15, 公開日: 2005-11-01, 最終更新日: 2024-03-13)

主引用文献

Dong, G.,Hutagalung, A.H.,Fu, C.,Novick, P.,Reinisch, K.M.
The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif
Nat.Struct.Mol.Biol., 12:1094-1100, 2005

PubMed Abstract: The exocyst is a large complex that is required for tethering vesicles at the final stages of the exocytic pathway in all eukaryotes. Here we present the structures of the Exo70p subunit of this complex and of the C-terminal domains of Exo84p, at 2.0-A and 2.85-A resolution, respectively. Exo70p forms a 160-A-long rod with a novel fold composed of contiguous alpha-helical bundles. The Exo84p C terminus also forms a long rod (80 A), which unexpectedly has the same fold as the Exo70p N terminus. Our structural results and our experimental observations concerning the interaction between Exo70p and other exocyst subunits or Rho3p GTPase are consistent with an architecture wherein exocyst subunits are composed of mostly helical modules strung together into long rods.

PubMed: 16249794
DOI: 10.1038/nsmb1017

実験手法

X-RAY DIFFRACTION (2 Å)