2AZJ

Crystal structure for the mutant D81C of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase

2AZJ の概要

• エントリーDOI: 10.2210/pdb2azj/pdb
• 関連するPDBエントリー: 2AZK
• 分子名称: Geranylgeranyl pyrophosphate synthetase (2 entities in total)
• 機能のキーワード: hexpps, trans-prenyltransferase, transferase
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66664.03

構造登録者

Sun, H.Y.,Ko, T.P.,Kuo, C.J.,Guo, R.T.,Chou, C.C.,Liang, P.H.,Wang, A.H.J. (登録日: 2005-09-11, 公開日: 2006-03-14, 最終更新日: 2024-05-29)

主引用文献

Sun, H.Y.,Ko, T.P.,Kuo, C.J.,Guo, R.T.,Chou, C.C.,Liang, P.H.,Wang, A.H.J.
Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures
J.Bacteriol., 187:8137-8148, 2005

PubMed Abstract: Hexaprenyl pyrophosphate synthase (HexPPs) from Sulfolobus solfataricus catalyzes the synthesis of trans-C(30)-hexaprenyl pyrophosphate (HexPP) by reacting two isopentenyl pyrophosphate molecules with one geranylgeranyl pyrophosphate. The crystal structure of the homodimeric C(30)-HexPPs resembles those of other trans-prenyltransferases, including farnesyl pyrophosphate synthase (FPPs) and octaprenyl pyrophosphate synthase (OPPs). In both subunits, 10 core helices are arranged about a central active site cavity. Leu164 in the middle of the cavity controls the product chain length. Two protein conformers are observed in the S. solfataricus HexPPs structure, and the major difference between them occurs in the flexible region of residues 84 to 100. Several helices (alphaI, alphaJ, alphaK, and part of alphaH) and the associated loops have high-temperature factors in one monomer, which may be related to the domain motion that controls the entrance to the active site. Different side chain conformations of Trp136 in two HexPPs subunits result in weaker hydrophobic interactions at the dimer interface, in contrast to the symmetric pi-pi stacking interactions of aromatic side chains found in FPPs and OPPs. Finally, the three-conformer switched model may explain the catalytic process for HexPPs.

PubMed: 16291686
DOI: 10.1128/JB.187.23.8137-8148.2005

実験手法

X-RAY DIFFRACTION (2.4 Å)