2AZ0

Flock House virus B2-dsRNA Complex (P212121)

Summary for 2AZ0

• Entry DOI: 10.2210/pdb2az0/pdb
• Related: 2AZ2
• Descriptor: 5'-R(*GP*CP*AP*(5BU)P*GP*GP*AP*CP*GP*CP*GP*(5BU)P*CP*CP*AP*(5BU)P*GP*C)-3', B2 protein (3 entities in total)
• Functional Keywords: protein-rna complex, four-helix bundle, viral protein-rna complex, viral protein/rna
• Biological source: Flock house virus
• Total number of polymer chains: 4
• Total formula weight: 28073.05

Authors

Chao, J.A.,Lee, J.H.,Chapados, B.R.,Debler, E.W.,Schneemann, A.,Williamson, J.R. (deposition date: 2005-09-09, release date: 2005-10-11, Last modification date: 2024-02-14)

Primary citation

Chao, J.A.,Lee, J.H.,Chapados, B.R.,Debler, E.W.,Schneemann, A.,Williamson, J.R.
Dual modes of RNA-silencing suppression by Flock House virus protein B2.
Nat.Struct.Mol.Biol., 12:952-957, 2005

PubMed Abstract: As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix bundle that binds to one face of an A-form RNA duplex independently of sequence. These results suggest that B2 blocks both cleavage of the FHV genome by Dicer and incorporation of FHV small interfering RNAs into the RNA-induced silencing complex.

PubMed: 16228003
DOI: 10.1038/nsmb1005

Experimental method

X-RAY DIFFRACTION (2.6 Å)