2AXZ

Crystal structure of PrgX/cCF10 complex

2AXZ の概要

• エントリーDOI: 10.2210/pdb2axz/pdb
• 関連するPDBエントリー: 2AW6 2AWI 2AXU 2AXV
• 分子名称: PrgX, LVTLVFV peptide, TPPKEVT(MSE) peptide, ... (4 entities in total)
• 機能のキーワード: repressor, pheromone, dna binding, transcription
• 由来する生物種: Enterococcus faecalis
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 152995.61

構造登録者

Shi, K.,Brown, C.K.,Gu, Z.Y.,Kozlowicz, B.K.,Dunny, G.M.,Ohlendorf, D.H.,Earhart, C.A. (登録日: 2005-09-06, 公開日: 2005-12-06, 最終更新日: 2024-10-30)

主引用文献

Shi, K.,Brown, C.K.,Gu, Z.Y.,Kozlowicz, B.K.,Dunny, G.M.,Ohlendorf, D.H.,Earhart, C.A.
Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis.
Proc.Natl.Acad.Sci.Usa, 102:18596-18601, 2005

PubMed Abstract: Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled by intercellular signaling using small molecules. To date, understanding of the molecular mechanisms of peptide-mediated cell-cell signaling has been limited by a dearth of published information about the molecular structures of the signaling components. Here, we present the molecular structure of PrgX, a DNA- and peptide-binding protein that regulates expression of the conjugative transfer genes of the Enterococcus faecalis plasmid pCF10 in response to an intercellular peptide pheromone signal. Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that pheromone binding destabilizes PrgX tetramers, opening a 70-bp pCF10 DNA loop required for conjugation repression.

PubMed: 16339309
DOI: 10.1073/pnas.0506163102

実験手法

X-RAY DIFFRACTION (3 Å)