2AWI

Structure of PrgX Y153C mutant

Summary for 2AWI

• Entry DOI: 10.2210/pdb2awi/pdb
• Related: 2AW6 2AXU 2AXV 2AXZ
• Descriptor: PrgX (2 entities in total)
• Functional Keywords: repressor, pheromone, dna binding, regulatory domain, transcription
• Biological source: Enterococcus faecalis
• Total number of polymer chains: 12
• Total formula weight: 447181.08

Authors

Shi, K.,Brown, C.K.,Gu, Z.Y.,Kozlowicz, B.k.,Dunny, G.M.,Ohlendorf, D.H.,Earhart, C.A. (deposition date: 2005-09-01, release date: 2005-12-06, Last modification date: 2021-10-20)

Primary citation

Shi, K.,Brown, C.K.,Gu, Z.Y.,Kozlowicz, B.K.,Dunny, G.M.,Ohlendorf, D.H.,Earhart, C.A.
Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis.
Proc.Natl.Acad.Sci.Usa, 102:18596-18601, 2005

PubMed Abstract: Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled by intercellular signaling using small molecules. To date, understanding of the molecular mechanisms of peptide-mediated cell-cell signaling has been limited by a dearth of published information about the molecular structures of the signaling components. Here, we present the molecular structure of PrgX, a DNA- and peptide-binding protein that regulates expression of the conjugative transfer genes of the Enterococcus faecalis plasmid pCF10 in response to an intercellular peptide pheromone signal. Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that pheromone binding destabilizes PrgX tetramers, opening a 70-bp pCF10 DNA loop required for conjugation repression.

PubMed: 16339309
DOI: 10.1073/pnas.0506163102

Experimental method

X-RAY DIFFRACTION (2.25 Å)