2AUK

Structure of E. coli RNA polymerase beta' G/G' insert

2AUK の概要

• エントリーDOI: 10.2210/pdb2auk/pdb
• 関連するPDBエントリー: 2AUJ
• 分子名称: DNA-directed RNA polymerase beta' chain (2 entities in total)
• 機能のキーワード: sandwich-barrel hybrid motif, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 100683.30

構造登録者

Chlenov, M.,Masuda, S.,Murakami, K.S.,Nikiforov, V.,Darst, S.A.,Mustaev, A. (登録日: 2005-08-28, 公開日: 2005-10-04, 最終更新日: 2024-02-14)

主引用文献

Chlenov, M.,Masuda, S.,Murakami, K.S.,Nikiforov, V.,Darst, S.A.,Mustaev, A.
Structure and Function of Lineage-specific Sequence Insertions in the Bacterial RNA Polymerase beta' Subunit
J.Mol.Biol., 353:138-154, 2005

PubMed Abstract: The large beta and beta' subunits of the bacterial core RNA polymerase (RNAP) are highly conserved throughout evolution. Nevertheless, large sequence insertions in beta and beta' characterize specific evolutionary lineages of bacteria. The Thermus aquaticus RNAP beta' subunit contains a 283 residue insert between conserved regions A and B that is found in only four bacterial species. The Escherichia coli RNAP beta' subunit contains a 188 residue insert in the middle of conserved region G that is found in a wide range of bacterial species. Here, we present structural studies of these two beta' insertions. We show that the inserts comprise repeats of a previously characterized fold, the sandwich-barrel hybrid motif (as predicted from previous sequence analysis) and that the inserts serve significant roles in facilitating protein/protein and/or protein/nucleic acid interactions.

PubMed: 16154587
DOI: 10.1016/j.jmb.2005.07.073

実験手法

X-RAY DIFFRACTION (2.3 Å)