2AS9

Functional and structural characterization of Spl proteases from staphylococcus aureus

2AS9 の概要

• エントリーDOI: 10.2210/pdb2as9/pdb
• 関連するPDBエントリー: 1AGJ 1QY6
• 分子名称: serine protease, ZINC ION (3 entities in total)
• 機能のキーワード: serine protease, trypsin-like fold, staphylococcus aureus, hydrolase
• 由来する生物種: Staphylococcus aureus
• 細胞内の位置: Secreted : Q53782
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47209.96

構造登録者

Popowicz, G.M.,Dubin, G.,Stec-Niemczyk, J.,Czarny, A.,Dubin, A.,Potempa, J.,Holak, T.A. (登録日: 2005-08-23, 公開日: 2005-09-06, 最終更新日: 2024-03-13)

主引用文献

Popowicz, G.M.,Dubin, G.,Stec-Niemczyk, J.,Czarny, A.,Dubin, A.,Potempa, J.,Holak, T.A.
Functional and Structural Characterization of Spl Proteases from Staphylococcus aureus
J.Mol.Biol., 358:270-279, 2006

PubMed Abstract: Staphylococcus aureus is the major cause of nosocomial infections world-wide, with increasing prevalence of community-acquired diseases. The recent dramatic increase in multi-antibiotic resistance, including resistance to the last-resort drug, vancomycin, together with the lack of an effective vaccine highlight the need for better understanding of S.aureus pathogenicity. Comparative analysis of available bacterial genomes allows for the identification of previously uncharacterized S.aureus genes with potential roles in pathogenicity. A good example is a cluster of six serine protease-like (spl) genes encompassed in one operon, which encode for putative proteases with similarity to staphylococcal glutamylendopeptidase (V8 protease). Here, we describe an efficient expression system for the production of recombinant SplB and SplC proteases in Escherichia coli, together with structural and functional characterization of the purified enzymes. A unique mechanism of cytoplasm protection against activity of misdirected SplB was uncovered. Apparently, the co-translated signal peptide maintains protease latency until it is cleaved by the signal peptidase during protein secretion. Furthermore, the crystal structure of the SplC protease revealed a fold resembling that of the V8 protease and epidermolytic toxins. Arrangement of the active site cleft and substrate-binding pocket of SplC explains the mechanism of enzyme latency and suggests that some Spl proteases possess restricted substrate specificity similar to that of the V8 protease and epidermolytic toxins.

PubMed: 16516230
DOI: 10.1016/j.jmb.2006.01.098

実験手法

X-RAY DIFFRACTION (1.7 Å)