2AQA

NMR structural analysis of Nop10p from Saccharomyces cerevisiae

Summary for 2AQA

• Entry DOI: 10.2210/pdb2aqa/pdb
• NMR Information: BMRB: 6846
• Descriptor: H/ACA ribonucleoprotein complex subunit 3 (1 entity in total)
• Functional Keywords: nop10p, rna binding protein
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Nucleus, nucleolus: Q6Q547
• Total number of polymer chains: 1
• Total formula weight: 7347.43

Authors

Hamma, T.,Reichow, S.L.,Varani, G.,Ferre-D'Amare, A.R. (deposition date: 2005-08-17, release date: 2005-11-15, Last modification date: 2024-05-22)

Primary citation

Hamma, T.,Reichow, S.L.,Varani, G.,Ferre-D'Amare, A.R.
The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs.
Nat.Struct.Mol.Biol., 12:1101-1107, 2005

PubMed Abstract: Box H/ACA ribonucleoprotein particles (RNPs) catalyze RNA pseudouridylation and direct processing of ribosomal RNA, and are essential architectural components of vertebrate telomerases. H/ACA RNPs comprise four proteins and a multihelical RNA. Two proteins, Cbf5 and Nop10, suffice for basal enzymatic activity in an archaeal in vitro system. We now report their cocrystal structure at 1.95-A resolution. We find that archaeal Cbf5 can assemble with yeast Nop10 and with human telomerase RNA, consistent with the high sequence identity of the RNP components between archaea and eukarya. Thus, the Cbf5-Nop10 architecture is phylogenetically conserved. The structure shows how Nop10 buttresses the active site of Cbf5, and it reveals two basic troughs that bidirectionally extend the active site cleft. Mutagenesis results implicate an adjacent basic patch in RNA binding. This tripartite RNA-binding surface may function as a molecular bracket that organizes the multihelical H/ACA and telomerase RNAs.

PubMed: 16286935
DOI: 10.1038/nsmb1036

Experimental method

SOLUTION NMR