2AJF

Structure of SARS coronavirus spike receptor-binding domain complexed with its receptor

2AJF の概要

• エントリーDOI: 10.2210/pdb2ajf/pdb
• 分子名称: Angiotensin-converting enzyme-Related Carboxypeptidase (Ace2), SARS-coronavirus spike protein, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: antiparallel beta sheet, extended loop, hydrolase-viral protein complex, hydrolase/viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 182055.53

構造登録者

Li, F.,Li, W.,Farzan, M.,Harrison, S.C. (登録日: 2005-08-01, 公開日: 2005-09-20, 最終更新日: 2024-12-25)

主引用文献

Li, F.,Li, W.,Farzan, M.,Harrison, S.C.
Structure of SARS coronavirus spike receptor-binding domain complexed with receptor.
Science, 309:1864-1868, 2005

PubMed Abstract: The spike protein (S) of SARS coronavirus (SARS-CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The crystal structure at 2.9 angstrom resolution of the RBD bound with the peptidase domain of human ACE2 shows that the RBD presents a gently concave surface, which cradles the N-terminal lobe of the peptidase. The atomic details at the interface between the two proteins clarify the importance of residue changes that facilitate efficient cross-species infection and human-to-human transmission. The structure of the RBD suggests ways to make truncated disulfide-stabilized RBD variants for use in the design of coronavirus vaccines.

PubMed: 16166518
DOI: 10.1126/science.1116480

実験手法

X-RAY DIFFRACTION (2.9 Å)