2AI6

Solution structure of human phosphohistidine phosphatase 1

2AI6 の概要

• エントリーDOI: 10.2210/pdb2ai6/pdb
• NMR情報: BMRB: 6625
• 分子名称: 14 kDa phosphohistidine phosphatase (1 entity in total)
• 機能のキーワード: alpha/beta architecture, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13851.52

構造登録者

Gong, W.,Cui, G.,Jin, C.,Xia, B. (登録日: 2005-07-29, 公開日: 2006-10-17, 最終更新日: 2024-05-01)

主引用文献

Gong, W.,Li, Y.,Cui, G.,Hu, J.,Fang, H.,Jin, C.,Xia, B.
Solution structure and catalytic mechanism of human protein histidine phosphatase 1.
Biochem.J., 418:337-344, 2009

PubMed Abstract: Protein histidine phosphorylation exists widely in vertebrates, and it plays important roles in signal transduction and other cellular functions. However, knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited. To date, only one vertebrate PHPT has been discovered, and two crystal structures of hPHPT1 (human PHPT1) have been solved. However, these two structures gave different ligand-binding sites and co-ordination patterns. In the present paper, we have solved the solution structures of hPHPT1 in both P(i)-free and P(i)-bound states. Through comparison of the structures, along with a mutagenesis study, we have determined the active site of hPHPT1. In contrast with previous results, our results indicate that the active site is located between helix alpha1 and loop L5. His(53) was identified to be the catalytic residue, and the NH groups of residues His(53), Ala(54) and Ala(96) and the OH group of Ser(94) should act as anchors of P(i) or substrate by forming H-bonds with P(i). On the basis of our results, a catalytic mechanism is proposed for hPHPT1: the imidazole ring of His(53) serves as a general base to activate a water molecule, and the activated water would attack the substrate as a nucleophile in the catalysis; the positively charged side chain of Lys(21) can help stabilize the transition state. No similar catalytic mechanism can be found in the EzCatDB database.

PubMed: 18991813
DOI: 10.1042/BJ20081571

実験手法

SOLUTION NMR