2AI3

Purine nucleoside phosphorylase from calf spleen

2AI3 の概要

• エントリーDOI: 10.2210/pdb2ai3/pdb
• 関連するPDBエントリー: 2AI1 2AI2
• 分子名称: Purine nucleoside phosphorylase, MAGNESIUM ION, ZINC ION, ... (6 entities in total)
• 機能のキーワード: purine nucleoside phosphorylase, multisubstrate analog inhibitor, transferase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Cytoplasm, cytoskeleton (By similarity): P55859
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32714.63

構造登録者

Toms, A.V.,Wang, W.,Li, Y.,Ganem, B.,Ealick, S.E. (登録日: 2005-07-28, 公開日: 2005-10-25, 最終更新日: 2023-08-23)

主引用文献

Toms, A.V.,Wang, W.,Li, Y.,Ganem, B.,Ealick, S.E.
Novel multisubstrate inhibitors of mammalian purine nucleoside phosphorylase.
Acta Crystallogr.,Sect.D, 61:1449-1458, 2005

PubMed Abstract: In an effort to develop potent multisubstrate-analog inhibitors of purine nucleoside phosphorylase (PNP), three nucleoside phosphonates were designed utilizing structural information from the previously reported structures of complexes of bovine PNP with substrates and products. The nucleoside phosphonates contain an acetal linkage at the O2' and O3' positions and a two-C-atom spacer between the ribose and phosphate moieties. The linkage enables the compounds to simultaneously occupy the purine-, ribose- and phosphate-binding sites. The chemical syntheses, inhibition profiles and structural characterization of these novel multisubstrate analog inhibitors with bovine PNP are described.

PubMed: 16239721
DOI: 10.1107/S0907444905025503

実験手法

X-RAY DIFFRACTION (1.7 Å)