2ADB

Solution structure of Polypyrimidine Tract Binding protein RBD2 complexed with CUCUCU RNA

2ADB の概要

• エントリーDOI: 10.2210/pdb2adb/pdb
• 関連するPDBエントリー: 2AD9 2ADC
• 分子名称: 5'-R(*CP*UP*CP*UP*CP*U)-3', Polypyrimidine tract-binding protein 1 (2 entities in total)
• 機能のキーワード: rbd, rrm, protein-rna complex, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P26599
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18167.62

構造登録者

Oberstrass, F.C.,Auweter, S.D.,Erat, M.,Hargous, Y.,Henning, A.,Wenter, P.,Reymond, L.,Pitsch, S.,Black, D.L.,Allain, F.H.T. (登録日: 2005-07-20, 公開日: 2005-10-04, 最終更新日: 2024-05-29)

主引用文献

Oberstrass, F.C.,Auweter, S.D.,Erat, M.,Hargous, Y.,Henning, A.,Wenter, P.,Reymond, L.,Amir-Ahmady, B.,Pitsch, S.,Black, D.L.,Allain, F.H.T.
Structure of PTB bound to RNA: specific binding and implications for splicing regulation
Science, 309:2054-2057, 2005

PubMed Abstract: The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor.

PubMed: 16179478
DOI: 10.1126/science.1114066

実験手法

SOLUTION NMR