2AA1

Crystal structure of the cathodic hemoglobin isolated from the Antarctic fish Trematomus Newnesi

2AA1 の概要

• エントリーDOI: 10.2210/pdb2aa1/pdb
• 分子名称: Hemoglobin alpha-1 chain, Hemoglobin beta-C chain, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: hemoglobin, root effect, cooperativity, antarctic fish, oxygen storage-transport complex, oxygen storage/transport
• 由来する生物種: Trematomus newnesi (dusky notothen); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 66427.72

構造登録者

Mazzarella, L.,Bonomi, G.,Lubrano, M.C.,Merlino, A.,Riccio, A.,Vergara, A.,Vitagliano, L.,Verde, C.,Di Prisco, G. (登録日: 2005-07-13, 公開日: 2005-08-02, 最終更新日: 2024-10-16)

主引用文献

Mazzarella, L.,Bonomi, G.,Lubrano, M.C.,Merlino, A.,Riccio, A.,Vergara, A.,Vitagliano, L.,Verde, C.,di Prisco, G.
Minimal structural requirements for root effect: crystal structure of the cathodic hemoglobin isolated from the antarctic fish Trematomus newnesi
Proteins, 62:316-321, 2006

PubMed Abstract: The cathodic hemoglobin component of the Antarctic fish Trematomus newnesi (HbCTn) is a Root-effect protein. The interpretation of its functional properties in relation to its sequence is puzzling. Indeed, HbCTn sequence is characterized by an extremely low histidyl content, and in particular by the lack of His146beta and His69beta, which are believed to be important in Bohr and Root effects, respectively. Furthermore, previous analyses suggested that the local environment of Asp95alpha, Asp99beta, and Asp101beta should not be appropriate for the formation of Asp-Asp interactions, which are important for the Root effect. Here, we report the high-resolution crystal structure of the deoxy form of HbCTn. Our data provide a structural interpretation for the very low oxygen affinity of the protein and insights into the structural determinants of the Root effect protein. The structure demonstrates that the presence of Ile41alpha and Ser97alpha at the alpha1beta2 interface does not prevent the formation of the inter-Asp interactions in HbCTn, as previous studies had suggested. The present data indicate that the hydrogen bond formed between Asp95alpha and Asp101beta, which is stabilized by Asp99beta, is per se sufficient to generate the Root effect, and it is the minimal structural requirement needed for the design of Root-effect Hbs.

PubMed: 16299734
DOI: 10.1002/prot.20709

実験手法

X-RAY DIFFRACTION (1.8 Å)