2A9L

SOLUTION NMR STRUCTURE OF A SUBSTRATE FOR THE ARCHAEAL PRE-TRNA SPLICING ENDONUCLEASES: THE BULGE-HELIX-BULGE MOTIF, MINIMIZED AVERAGE STRUCTURE

2A9L の概要

• エントリーDOI: 10.2210/pdb2a9l/pdb
• 分子名称: ABISS7 RNA (1 entity in total)
• 機能のキーワード: archaeal bulge-helix-bulge motif, pre-trna splicing, archaea, rna structure, ribonucleic acid, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12317.42

構造登録者

Diener, J.L.,Moore, P.B. (登録日: 1998-04-07, 公開日: 1998-06-17, 最終更新日: 2024-05-22)

主引用文献

Diener, J.L.,Moore, P.B.
Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif.
Mol.Cell, 1:883-894, 1998

PubMed Abstract: The structure of the bulge-helix-bulge motif that constitutes the intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy. The conformations of the two 3 nt bulges, where the pre-tRNA is cleaved, are stabilized by stacking interactions between bulge nucleotides and bases in the adjacent Watson-Crick helices and by a network of backbone hydrogen bonds. Both bulges are presented on the same minor groove face of the central 4 bp helix, and the overall structure has approximate two-fold symmetry, which makes it well-suited for attack by archaeal splicing endonucleases, which are symmetric dimers.

PubMed: 9660971
DOI: 10.1016/S1097-2765(00)80087-8

実験手法

SOLUTION NMR