2A90

Crystal Structure of the tandem WWE domain of Drosophila Deltex

2A90 の概要

• エントリーDOI: 10.2210/pdb2a90/pdb
• 分子名称: Deltex protein (2 entities in total)
• 機能のキーワード: wwe domain, metal binding protein
• 由来する生物種: Drosophila melanogaster (fruit fly)
• 細胞内の位置: Cytoplasm: Q23985
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26813.23

構造登録者

Zweifel, M.E.,Leahy, D.J.,Barrick, D. (登録日: 2005-07-10, 公開日: 2005-11-22, 最終更新日: 2024-11-13)

主引用文献

Zweifel, M.E.,Leahy, D.J.,Barrick, D.
Structure and Notch Receptor Binding of the Tandem WWE Domain of Deltex.
Structure, 13:1599-1611, 2005

PubMed Abstract: Deltex is a cytosolic effector of Notch signaling thought to bind through its N-terminal domain to the Notch receptor. Here we report the structure of the Drosophila Deltex N-terminal domain, which contains two tandem WWE sequence repeats. The WWE repeats, which adopt a novel fold, are related by an approximate two-fold axis of rotation. Although the WWE repeats are structurally distinct, they interact extensively and form a deep cleft at their junction that appears well suited for ligand binding. The two repeats are thermodynamically coupled; this coupling is mediated in part by a conserved segment that is immediately C-terminal to the second WWE domain. We demonstrate that although the Deltex WWE tandem is monomeric in solution, it forms a heterodimer with the ankyrin domain of the Notch receptor. These results provide structural and functional insight into how Deltex modulates Notch signaling, and how WWE modules recognize targets for ubiquitination.

PubMed: 16271883
DOI: 10.1016/j.str.2005.07.015

実験手法

X-RAY DIFFRACTION (2.15 Å)