2A6Q
Crystal structure of YefM-YoeB complex
Summary for 2A6Q
| Entry DOI | 10.2210/pdb2a6q/pdb |
| Related | 2A6R 2A6S |
| Descriptor | Antitoxin yefM, Toxin yoeB (3 entities in total) |
| Functional Keywords | yoeb, yefm, toxin, antitoxin, addiction modules, rnase, inhibitor, toxin inhibitor-toxin complex, toxin inhibitor/toxin |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 6 |
| Total formula weight | 58910.90 |
| Authors | Kamada, K.,Hanaoka, F. (deposition date: 2005-07-04, release date: 2005-08-23, Last modification date: 2024-03-13) |
| Primary citation | Kamada, K.,Hanaoka, F. Conformational Change in the Catalytic Site of the Ribonuclease YoeB Toxin by YefM Antitoxin Mol.Cell, 19:497-509, 2005 Cited by PubMed Abstract: The eubacterial chromosome encodes various addiction modules that control global levels of translation through RNA degradation. Crystal structures of the Escherichia coli YefM2 (antitoxin)-YoeB (toxin) complex and the free YoeB toxin have been determined. The structure of the heterotrimeric complex reveals an asymmetric disorder-to-order recognition strategy, in which one C terminus of the YefM homodimer exclusively interacts with an atypical microbial ribonuclease (RNase) fold of YoeB. Comparison with the YefM-free YoeB structure indicates a conformational rearrangement of the RNase catalytic site of YoeB, induced by interaction with YefM. Complementary biochemical experiments demonstrate that the YoeB toxin has an in vitro RNase activity that preferentially cleaves at the 3' end of purine ribonucleotides. PubMed: 16109374DOI: 10.1016/j.molcel.2005.07.004 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.05 Å) |
Structure validation
Download full validation report
