2A6Q

Crystal structure of YefM-YoeB complex

2A6Q の概要

• エントリーDOI: 10.2210/pdb2a6q/pdb
• 関連するPDBエントリー: 2A6R 2A6S
• 分子名称: Antitoxin yefM, Toxin yoeB (3 entities in total)
• 機能のキーワード: yoeb, yefm, toxin, antitoxin, addiction modules, rnase, inhibitor, toxin inhibitor-toxin complex, toxin inhibitor/toxin
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 58910.90

構造登録者

Kamada, K.,Hanaoka, F. (登録日: 2005-07-04, 公開日: 2005-08-23, 最終更新日: 2024-03-13)

主引用文献

Kamada, K.,Hanaoka, F.
Conformational Change in the Catalytic Site of the Ribonuclease YoeB Toxin by YefM Antitoxin
Mol.Cell, 19:497-509, 2005

PubMed Abstract: The eubacterial chromosome encodes various addiction modules that control global levels of translation through RNA degradation. Crystal structures of the Escherichia coli YefM2 (antitoxin)-YoeB (toxin) complex and the free YoeB toxin have been determined. The structure of the heterotrimeric complex reveals an asymmetric disorder-to-order recognition strategy, in which one C terminus of the YefM homodimer exclusively interacts with an atypical microbial ribonuclease (RNase) fold of YoeB. Comparison with the YefM-free YoeB structure indicates a conformational rearrangement of the RNase catalytic site of YoeB, induced by interaction with YefM. Complementary biochemical experiments demonstrate that the YoeB toxin has an in vitro RNase activity that preferentially cleaves at the 3' end of purine ribonucleotides.

PubMed: 16109374
DOI: 10.1016/j.molcel.2005.07.004

実験手法

X-RAY DIFFRACTION (2.05 Å)