2A62

Crystal structure of mouse cadherin-8 EC1-3

Summary for 2A62

• Entry DOI: 10.2210/pdb2a62/pdb
• Related: 1zvn 1zxk 2a4c 2a4e
• Descriptor: Cadherin-8, CALCIUM ION (2 entities in total)
• Functional Keywords: cadherin, extracellular domain, homodimer, calcium binding, cell adhesion
• Biological source: Mus musculus (house mouse)
• Cellular location: Cell membrane; Single-pass type I membrane protein: P97291
• Total number of polymer chains: 1
• Total formula weight: 35540.81

Authors

Patel, S.D.,Ciatto, C.,Chen, C.P.,Bahna, F.,Arkus, N.,Schieren, I.,Jessell, T.M.,Honig, B.,Price, S.R.,Shapiro, L. (deposition date: 2005-07-01, release date: 2006-04-25, Last modification date: 2024-02-14)

Primary citation

Patel, S.D.,Ciatto, C.,Chen, C.P.,Bahna, F.,Rajebhosale, M.,Arkus, N.,Schieren, I.,Jessell, T.M.,Honig, B.,Price, S.R.,Shapiro, L.
Type II cadherin ectodomain structures: implications for classical cadherin specificity.
Cell(Cambridge,Mass.), 124:1255-1268, 2006

PubMed Abstract: Type I and II classical cadherins help to determine the adhesive specificities of animal cells. Crystal-structure determination of ectodomain regions from three type II cadherins reveals adhesive dimers formed by exchange of N-terminal beta strands between partner extracellular cadherin-1 (EC1) domains. These interfaces have two conserved tryptophan side chains that anchor each swapped strand, compared with one in type I cadherins, and include large hydrophobic regions unique to type II interfaces. The EC1 domains of type I and type II cadherins appear to encode cell adhesive specificity in vitro. Moreover, perturbation of motor neuron segregation with chimeric cadherins depends on EC1 domain identity, suggesting that this region, which includes the structurally defined adhesive interface, encodes type II cadherin functional specificity in vivo.

PubMed: 16564015
DOI: 10.1016/j.cell.2005.12.046

Experimental method

X-RAY DIFFRACTION (4.5 Å)