2A43

Crystal Structure of a Luteoviral RNA Pseudoknot and Model for a Minimal Ribosomal Frameshifting Motif

2A43 の概要

• エントリーDOI: 10.2210/pdb2a43/pdb
• 分子名称: RNA Pseudoknot, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: plrv, potato leaf roll virus; bwyv, beet western yellow virus, pk, pseudoknot, rna., rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8388.68

構造登録者

Pallan, P.S.,Marshall, W.S.,Harp, J.,Jewett III, F.C.,Wawrzak, Z.,Brown II, B.A.,Rich, A.,Egli, M. (登録日: 2005-06-27, 公開日: 2005-09-27, 最終更新日: 2023-08-23)

主引用文献

Pallan, P.S.,Marshall, W.S.,Harp, J.,Jewett III, F.C.,Wawrzak, Z.,Brown II, B.A.,Rich, A.,Egli, M.
Crystal Structure of a Luteoviral RNA Pseudoknot and Model for a Minimal Ribosomal Frameshifting Motif
Biochemistry, 44:11315-11322, 2005

PubMed Abstract: To understand the role of structural elements of RNA pseudoknots in controlling the extent of -1-type ribosomal frameshifting, we determined the crystal structure of a high-efficiency frameshifting mutant of the pseudoknot from potato leaf roll virus (PLRV). Correlations of the structure with available in vitro frameshifting data for PLRV pseudoknot mutants implicate sequence and length of a stem-loop linker as modulators of frameshifting efficiency. Although the sequences and overall structures of the RNA pseudoknots from PLRV and beet western yellow virus (BWYV) are similar, nucleotide deletions in the linker and adjacent minor groove loop abolish frameshifting only with the latter. Conversely, mutant PLRV pseudoknots with up to four nucleotides deleted in this region exhibit nearly wild-type frameshifting efficiencies. The crystal structure helps rationalize the different tolerances for deletions in the PLRV and BWYV RNAs, and we have used it to build a three-dimensional model of the PRLV pseudoknot with a four-nucleotide deletion. The resulting structure defines a minimal RNA pseudoknot motif composed of 22 nucleotides capable of stimulating -1-type ribosomal frameshifts.

PubMed: 16114868
DOI: 10.1021/bi051061i

実験手法

X-RAY DIFFRACTION (1.34 Å)