2A2G

THE CRYSTAL STRUCTURES OF A2U-GLOBULIN AND ITS COMPLEX WITH A HYALINE DROPLET INDUCER.

2A2G の概要

• エントリーDOI: 10.2210/pdb2a2g/pdb
• 分子名称: PROTEIN (ALPHA-2U-GLOBULIN), D-LIMONENE 1,2-EPOXIDE (2 entities in total)
• 機能のキーワード: lipid-binding protein, lipid binding protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: 15. Secreted: P02761
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 83668.35

構造登録者

Chaudhuri, B.N.,Kleywegt, G.J.,Jones, T.A. (登録日: 1998-11-19, 公開日: 1999-08-13, 最終更新日: 2024-11-13)

主引用文献

Chaudhuri, B.N.,Kleywegt, G.J.,Bjorkman, J.,Lehman-McKeeman, L.D.,Oliver, J.D.,Jones, T.A.
The structures of alpha 2u-globulin and its complex with a hyaline droplet inducer.
Acta Crystallogr.,Sect.D, 55:753-762, 1999

PubMed Abstract: Alpha 2u-globulin (A2U) is the major urinary protein excreted by adult male rats. The structure of a monoclinic crystal form of A2U was reported in 1992 [Böcskei et al. (1992). Nature (London), 360, 186-188]. The structures of an orthorhombic crystal form of A2U at 2. 5 A resolution (refined to an R factor of 0.248; Rfree = 0.264) and of a complex between A2U and d-limonene 1,2-epoxide (DLO) at 2.9 A resolution (R factor = 0.248; Rfree = 0.260) are presented here. DLO is one of a diverse group of chemicals which cause a male rat-specific renal carcinogenesis called hyaline-droplet nephropathy. The rate-determining step in the development of this disorder is the binding of the toxin to A2U. Comparison of the cavities in A2U and in the corresponding mouse urinary protein (MUP) reveal that the former is tailor-made for small oval hydrophobic ligands such as DLO. The cavity in MUP is more shallow and elongated and cannot easily accommodate such ligands.

PubMed: 10089305
DOI: 10.1107/S0907444998017211

実験手法

X-RAY DIFFRACTION (2.9 Å)