2A15

X-ray Crystal Structure of RV0760 from Mycobacterium Tuberculosis at 1.68 Angstrom Resolution

2A15 の概要

• エントリーDOI: 10.2210/pdb2a15/pdb
• 分子名称: HYPOTHETICAL PROTEIN Rv0760c, NICOTINAMIDE (3 entities in total)
• 機能のキーワード: beta-alpha-barrel, structural genomics, psi, protein structure initiative, tb structural genomics consortium, tbsgc, unknown function
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15378.13

構造登録者

Garen, C.R.,Cherney, M.M.,James, M.N.G.,TB Structural Genomics Consortium (TBSGC) (登録日: 2005-06-17, 公開日: 2005-10-11, 最終更新日: 2024-04-03)

主引用文献

Cherney, M.M.,Garen, C.R.,James, M.N.G.
Crystal structure of Mycobacterium tuberculosis Rv0760c at 1.50 A resolution, a structural homolog of Delta(5)-3-ketosteroid isomerase
Biochim.Biophys.Acta, 1784:1625-1632, 2008

PubMed Abstract: We have determined the X-ray crystal structure of the Mycobacterium tuberculosis (Mtb) gene product encoded by the open reading frame Rv0760c at 1.50 A resolution by single-wavelength anomalous dispersion (SAD) phasing of diffraction data from crystals of the selenomethionine-substituted protein. Refinement against diffraction data from the native protein resulted in R(work)=19.5% and R(free)=21.4%. The X-ray crystal structure shows that the homodimeric Rv0760c polypeptide has an alpha + beta conical barrel fold placing it among many structural neighbors of the nuclear transport factor 2 family (NTF2). This family is highly conserved in terms of structure; however the substrates and individual protein functions are diverse. The structures of native Rv0760c in several different crystal forms and Rv0760c bound to 17beta-estradiol 17-hemisuccinate (EH) have also been solved and analyzed.

PubMed: 18589008
DOI: 10.1016/j.bbapap.2008.05.012

実験手法

X-RAY DIFFRACTION (1.68 Å)