2A11

Crystal Structure of Nuclease Domain of Ribonuclase III from Mycobacterium Tuberculosis

2A11 の概要

• エントリーDOI: 10.2210/pdb2a11/pdb
• 分子名称: Ribonuclease III, CALCIUM ION (3 entities in total)
• 機能のキーワード: ribonuclease; rnase iii; nuclease domain; structural genomics, psi, protein structure initiative; mycobacterium tuberculosis structural proteomics project (xmtb), transcription, translation, hydrolase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25637.14

構造登録者

Akey, D.L.,Berger, J.M.,Mycobacterium Tuberculosis Structural Proteomics Project (XMTB) (登録日: 2005-06-17, 公開日: 2005-07-05, 最終更新日: 2024-02-14)

主引用文献

Akey, D.L.,Berger, J.M.
Structure of the nuclease domain of ribonuclease III from M. tuberculosis at 2.1 A
Protein Sci., 14:2744-2750, 2005

PubMed Abstract: RNase III enzymes are a highly conserved family of proteins that specifically cleave double-stranded (ds)RNA. These proteins are involved in a diverse group of functions, including ribosomal RNA processing, mRNA maturation and decay, snRNA and snoRNA processing, and RNA interference. Here we report the crystal structure of the nuclease domain of RNase III from the pathogen Mycobacterium tuberculosis. Although globally similar to other RNase III folds, this structure has some features not observed in previously reported models. These include the presence of an additional metal ion near the catalytic site, as well as conserved secondary structural elements that are proposed to have functional roles in the recognition of dsRNAs.

PubMed: 16155207
DOI: 10.1110/ps.051665905

実験手法

X-RAY DIFFRACTION (2.1 Å)