2A0B

HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI

Summary for 2A0B

• Entry DOI: 10.2210/pdb2a0b/pdb
• Descriptor: HPT DOMAIN, ZINC ION (3 entities in total)
• Functional Keywords: sensory transduction, histidine kinase, phosphotransfer, two-component system, four-helix bundle
• Biological source: Escherichia coli
• Cellular location: Cell inner membrane; Multi-pass membrane protein: P22763
• Total number of polymer chains: 1
• Total formula weight: 14079.35

Authors

Kato, M.,Mizuno, T.,Shimizu, T.,Hakoshima, T. (deposition date: 1998-04-02, release date: 1998-06-17, Last modification date: 2024-02-14)

Primary citation

Kato, M.,Mizuno, T.,Shimizu, T.,Hakoshima, T.
Refined structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli at 1.57 A resolution.
Acta Crystallogr.,Sect.D, 55:1842-1849, 1999

PubMed Abstract: The crystal structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli has been refined to 1.57 A resolution, using the coordinates of the earlier 2.06 A structure as a starting model. The final model contained 956 protein atoms, one zinc ion and 156 water molecules, with an R factor of 19.0%. The high-resolution electron-density maps clearly revealed additional solvent molecules and seven discrete rotamers in the protein side chains. One residue, Met755, was fully buried but was able to occupy the space in the hydrophobic core by means of the two-state conformation of its side chain. One water molecule was buried in the protein core and contributed to the rigidity of the HPt domain, cooperating in the coordination of the zinc ion.

PubMed: 10531481
DOI: 10.1107/S0907444999010392

Experimental method

X-RAY DIFFRACTION (1.57 Å)