2A0B

HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI

2A0B の概要

• エントリーDOI: 10.2210/pdb2a0b/pdb
• 分子名称: HPT DOMAIN, ZINC ION (3 entities in total)
• 機能のキーワード: sensory transduction, histidine kinase, phosphotransfer, two-component system, four-helix bundle
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P22763
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14079.35

構造登録者

Kato, M.,Mizuno, T.,Shimizu, T.,Hakoshima, T. (登録日: 1998-04-02, 公開日: 1998-06-17, 最終更新日: 2024-02-14)

主引用文献

Kato, M.,Mizuno, T.,Shimizu, T.,Hakoshima, T.
Refined structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli at 1.57 A resolution.
Acta Crystallogr.,Sect.D, 55:1842-1849, 1999

PubMed Abstract: The crystal structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli has been refined to 1.57 A resolution, using the coordinates of the earlier 2.06 A structure as a starting model. The final model contained 956 protein atoms, one zinc ion and 156 water molecules, with an R factor of 19.0%. The high-resolution electron-density maps clearly revealed additional solvent molecules and seven discrete rotamers in the protein side chains. One residue, Met755, was fully buried but was able to occupy the space in the hydrophobic core by means of the two-state conformation of its side chain. One water molecule was buried in the protein core and contributed to the rigidity of the HPt domain, cooperating in the coordination of the zinc ion.

PubMed: 10531481
DOI: 10.1107/S0907444999010392

実験手法

X-RAY DIFFRACTION (1.57 Å)