2Y8R
Crystal structure of apo AMA1 mutant (Tyr230Ala) from Toxoplasma gondii
Summary for 2Y8R
| Entry DOI | 10.2210/pdb2y8r/pdb |
| Related | 2Y8S 2Y8T |
| Descriptor | APICAL MEMBRANE ANTIGEN, PUTATIVE, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total) |
| Functional Keywords | membrane protein, moving junction, invasion |
| Biological source | TOXOPLASMA GONDII |
| Total number of polymer chains | 4 |
| Total formula weight | 192570.28 |
| Authors | Tonkin, M.L.,Roques, M.,Lamarque, M.H.,Pugniere, M.,Douguet, D.,Crawford, J.,Lebrun, M.,Boulanger, M.J. (deposition date: 2011-02-10, release date: 2011-08-03, Last modification date: 2020-07-29) |
| Primary citation | Tonkin, M.L.,Roques, M.,Lamarque, M.H.,Pugniere, M.,Douguet, D.,Crawford, J.,Lebrun, M.,Boulanger, M.J. Host Cell Invasion by Apicomplexan Parasites: Insights from the Co-Structure of Ama1 with a Ron2 Peptide Science, 333:463-, 2011 Cited by PubMed Abstract: Apicomplexan parasites such as Toxoplasma gondii and Plasmodium species actively invade host cells through a moving junction (MJ) complex assembled at the parasite-host cell interface. MJ assembly is initiated by injection of parasite rhoptry neck proteins (RONs) into the host cell, where RON2 spans the membrane and functions as a receptor for apical membrane antigen 1 (AMA1) on the parasite. We have determined the structure of TgAMA1 complexed with a RON2 peptide at 1.95 angstrom resolution. A stepwise assembly mechanism results in an extensive buried surface area, enabling the MJ complex to resist the mechanical forces encountered during host cell invasion. Besides providing insights into host cell invasion by apicomplexan parasites, the structure offers a basis for designing therapeutics targeting these global pathogens. PubMed: 21778402DOI: 10.1126/SCIENCE.1204988 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.45 Å) |
Structure validation
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