2XBO

Equine Rhinitis A Virus in Complex with its Sialic Acid Receptor

Summary for 2XBO

• Entry DOI: 10.2210/pdb2xbo/pdb
• Related: 2WFF 2WS9
• Related PRD ID: PRD_900025
• Descriptor: P1, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: virus, capsid
• Biological source: EQUINE RHINITIS A VIRUS; More
• Total number of polymer chains: 4
• Total formula weight: 85902.34

Authors

Fry, E.E.,Tuthill, T.J.,Harlos, K.,Walter, T.S.,Rowlands, D.J.,Stuart, D.I. (deposition date: 2010-04-14, release date: 2010-09-22, Last modification date: 2024-05-08)

Primary citation

Fry, E.E.,Tuthill, T.J.,Harlos, K.,Walter, T.S.,Rowlands, D.J.,Stuart, D.I.
The Crystal Structure of Equine Rhinitis a Virus in Complex with its Sialic Acid Receptor.
J.Gen.Virol., 91:1971-, 2010

PubMed Abstract: Equine rhinitis A virus (ERAV) shares many features with foot-and-mouth disease virus (FMDV) and both are classified within the genus Aphthovirus of the family Picornaviridae. ERAV is used as a surrogate for FMDV research as it does not require high-level biosecurity. In contrast to FMDV, which uses integrins as cellular receptors, the receptor for ERAV has been reported to involve the sugar moiety sialic acid. This study confirmed the importance of sialic acid for cell entry by ERAV and reports the crystal structure of ERAV particles complexed with the receptor analogue 3'-sialyllactose. The receptor is attached to the rim of a capsid pit adjacent to the major immunogenic site and distinct from the sialic acid binding site used by a related picornavirus, the cardiovirus Theiler's murine encephalitis virus. The structure of the major antigenic determinant of the virus, previously identified from antibody escape mutations, is also described as the EF loop of VP1, which forms a hairpin stretching across the capsid surface close to the icosahedral fivefold axis, neighbouring the receptor-binding site, and spanning two protomeric units.

PubMed: 20427563
DOI: 10.1099/VIR.0.020420-0

Experimental method

X-RAY DIFFRACTION (4 Å)