2W6E
Low resolution structures of bovine mitochondrial F1-ATPase during controlled dehydration:hydration state 1.
Summary for 2W6E
| Entry DOI | 10.2210/pdb2w6e/pdb |
| Related | 1BMF 1COW 1E1Q 1E1R 1E79 1EFR 1H8E 1H8H 1NBM 1OHH 1QO1 1W0J 1W0K 2CK3 2JDI 2JIZ 2JJ1 2JJ2 2V7Q 2W6F 2W6G 2W6H 2W6I 2W6J |
| Descriptor | ATP SYNTHASE SUBUNIT ALPHA HEART ISOFORM, MITOCHONDRIAL, ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL, ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL, ... (4 entities in total) |
| Functional Keywords | atp phosphorylase (h+ transporting), transit peptide, f1fo atp phosphorylase, ion transport, atp synthesis, ubl conjugation, cf(1), p-loop, hydrolase, hydrogen ion transport, pyrrolidone carboxylic acid |
| Biological source | BOS TAURUS (BOVINE) More |
| Cellular location | Mitochondrion inner membrane (By similarity): P19483 Mitochondrion: P00829 P05631 |
| Total number of polymer chains | 7 |
| Total formula weight | 381953.31 |
| Authors | Sanchez-Weatherby, J.,Felisaz, F.,Gobbo, A.,Huet, J.,Ravelli, R.B.G.,Bowler, M.W.,Cipriani, F. (deposition date: 2008-12-18, release date: 2009-10-27, Last modification date: 2023-12-13) |
| Primary citation | Sanchez-Weatherby, J.,Felisaz, F.,Gobbo, A.,Huet, J.,Ravelli, R.B.G.,Bowler, M.W.,Cipriani, F. Improving Diffraction by Humidity Control: A Novel Device Compatible with X-Ray Beamlines. Acta Crystallogr.,Sect.D, 65:1237-, 2009 Cited by PubMed Abstract: Dehydration of protein crystals is rarely used, despite being a post-crystallization method that is useful for the improvement of crystal diffraction properties, as it is difficult to reproduce and monitor. A novel device for hydration control of macromolecular crystals in a standard data-collection environment has been developed. The device delivers an air stream of precise relative humidity that can be used to alter the amount of water in macromolecular crystals. The device can be rapidly installed and is fully compatible with most standard synchrotron X-ray beamlines. Samples are mounted in cryoloops and the progress of dehydration can be monitored both optically and by the acquisition of diffraction images. Once the optimal hydration level has been obtained, cryocooling is easy to achieve by hand or by using a sample changer. The device has been thoroughly tested on several ESRF beamlines and is available to users. PubMed: 19966409DOI: 10.1107/S0907444909037822 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (6.5 Å) |
Structure validation
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