2RRL

Solution structure of the C-terminal domain of the FliK

Summary for 2RRL

• Entry DOI: 10.2210/pdb2rrl/pdb
• NMR Information: BMRB: 11423
• Descriptor: Flagellar hook-length control protein (1 entity in total)
• Functional Keywords: flik, flhb, bacterial flagella motor, hook-length control, protein transport
• Biological source: Salmonella typhimurium
• Total number of polymer chains: 1
• Total formula weight: 17773.78

Authors

Mizuno, S.,Tate, S.,Kobayashi, N.,Amida, H. (deposition date: 2011-01-02, release date: 2011-05-18, Last modification date: 2024-05-29)

Primary citation

Mizuno, S.,Amida, H.,Kobayashi, N.,Aizawa, S.,Tate, S.
The NMR structure of FliK, the trigger for the switch of substrate specificity in the flagellar type III secretion apparatus
J.Mol.Biol., 409:558-573, 2011

PubMed Abstract: The flagellar cytoplasmic protein FliK controls hook elongation by two successive events: by determining hook length and by stopping the supply of hook protein. These two distinct roles are assigned to different parts of FliK: the N-terminal half (FliK(N)) determines length and the C-terminal half (FliK(C)) switches secretion from the hook protein to the filament protein. The interaction of FliK(C) with FlhB, the switchable secretion gate, triggers the switch. By NMR spectroscopy, we demonstrated that FliK is largely unstructured and determined the structure of a compact domain in FliK(C). The compact domain, denoted the FliK(C) core domain, consists of two α-helices, a β-sheet with two parallel and two antiparallel strands, and several exposed loops. Based on the functional data obtained by a series of deletion mutants of the FliK(C) core domain, we constructed a model of the complex between the FliK(C) core domain and FlhB(C). The model suggested that one of the FliK(C) loops has a high probability of interacting with the C-terminal domain of FlhB (FlhB(C)) as the FliK molecule enters the secretion gate. We suggest that the autocleaved NPTH sequence in FlhB contacts loop 2 of FliK(C) to trigger the switching event. This contact is sterically prevented when NPTH is not cleaved. Thus, the structure of FliK provides insight into the mechanism by which this bifunctional protein triggers a switch in the export of substrates.

PubMed: 21510958
DOI: 10.1016/j.jmb.2011.04.008

Experimental method

SOLUTION NMR