2RPS

Solution structure of a novel insect chemokine isolated from integument

Summary for 2RPS

• Entry DOI: 10.2210/pdb2rps/pdb
• NMR Information: BMRB: 11054
• Descriptor: Chemokine (1 entity in total)
• Functional Keywords: immune system
• Biological source: Pseudaletia separata (Mythimna separata, Oriental armyworm)
• Total number of polymer chains: 1
• Total formula weight: 3545.01

Authors

Kamiya, M.,Nakatogawa, S.,Oda, Y.,Kamijima, T.,Aizawa, T.,Demura, M.,Hayakawa, Y.,Kawano, K. (deposition date: 2008-07-28, release date: 2009-06-16, Last modification date: 2024-11-20)

Primary citation

Nakatogawa, S.,Oda, Y.,Kamiya, M.,Kamijima, T.,Aizawa, T.,Clark, K.D.,Demura, M.,Kawano, K.,Strand, M.R.,Hayakawa, Y.
A novel peptide mediates aggregation and migration of hemocytes from an insect
Curr.Biol., 19:779-785, 2009

PubMed Abstract: Insect blood cells (hemocytes) comprise an essential arm of the immune system [1-7]. Several factors mediating recognition and phagocytosis of foreign intruders by hemocytes have been identified, but the mechanisms regulating hemocyte movement remain fragmentary. Embryonic hemocytes from Drosophila migrate along stereotypical routes in response to chemotactic signals from PVF ligands, members of the platelet-derived growth factor family [8-12]. Embryonic and larval hemocytes also accumulate at external wounds [11-13], but PVFs are not required for this response, suggesting involvement by other, unknown factors. Here we report the identification of hemocyte chemotactic peptide (HCP) from the moth Pseudaletia separata and present evidence that it stimulates aggregation and directed movement of phagocytic hemocytes. Spatiotemporal studies revealed that HCP is expressed in both epidermal cells and hemocytes, whereas structure-function studies identified post-translational modifications important for activity. HCP also shares similarities with another group of cytokines from moths called ENF peptides [14-17]. Taken together, our results identify HCP as a chemotactic cytokine that enhances clotting at wound sites in larvae.

PubMed: 19375321
DOI: 10.1016/j.cub.2009.03.050

Experimental method

SOLUTION NMR