2NYJ

Crystal structure of the ankyrin repeat domain of TRPV1

Summary for 2NYJ

• Entry DOI: 10.2210/pdb2nyj/pdb
• Descriptor: Transient receptor potential cation channel subfamily V member 1, ADENOSINE-5'-TRIPHOSPHATE (2 entities in total)
• Functional Keywords: trpv1, ankyrin repeat domain, transport protein
• Biological source: Rattus norvegicus (Norway rat)
• Cellular location: Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein : O35433
• Total number of polymer chains: 1
• Total formula weight: 30946.43

Authors

Jin, X.,Gaudet, R. (deposition date: 2006-11-20, release date: 2007-07-03, Last modification date: 2023-12-27)

Primary citation

Lishko, P.V.,Procko, E.,Jin, X.,Phelps, C.B.,Gaudet, R.
The Ankyrin Repeats of TRPV1 Bind Multiple Ligands and Modulate Channel Sensitivity.
Neuron, 54:905-918, 2007

PubMed Abstract: TRPV1 plays a key role in nociception, as it is activated by heat, low pH, and ligands such as capsaicin, leading to a burning pain sensation. We describe the structure of the cytosolic ankyrin repeat domain (ARD) of TRPV1 and identify a multiligand-binding site important in regulating channel sensitivity within the TRPV1-ARD. The structure reveals a binding site that accommodates triphosphate nucleotides such as ATP, and biochemical studies demonstrate that calmodulin binds the same site. Electrophysiology experiments show that either ATP or PIP2 prevent desensitization to repeated applications of capsaicin, i.e., tachyphylaxis, while calmodulin plays an opposing role and is necessary for tachyphylaxis. Mutations in the TRPV1-ARD binding site eliminate tachyphylaxis. We present a model for the calcium-dependent regulation of TRPV1 via competitive interactions of ATP and calmodulin at the TRPV1-ARD-binding site and discuss its relationship to the C-terminal region previously implicated in interactions with PIP2 and calmodulin.

PubMed: 17582331
DOI: 10.1016/j.neuron.2007.05.027

Experimental method

X-RAY DIFFRACTION (3.2 Å)