2N7S

Solution Structure of Leptospiral LigA4 Big Domain

Replaces:  2N2I

Summary for 2N7S

• Entry DOI: 10.2210/pdb2n7s/pdb
• NMR Information: BMRB: 25819
• Descriptor: Ig-like repeat domain protein 1 (1 entity in total)
• Functional Keywords: pdz, structural protein
• Biological source: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
• Total number of polymer chains: 1
• Total formula weight: 9481.26

Authors

Mei, S. (deposition date: 2015-09-17, release date: 2015-10-21, Last modification date: 2024-05-15)

Primary citation

Mei, S.,Zhang, J.,Zhang, X.,Tu, X.
Solution structure of leptospiral LigA4 Big domain.
Biochem. Biophys. Res. Commun., 467:288-292, 2015

PubMed Abstract: Pathogenic Leptospiraspecies express immunoglobulin-like proteins which serve as adhesins to bind to the extracellular matrices of host cells. Leptospiral immunoglobulin-like protein A (LigA), a surface exposed protein containing tandem repeats of bacterial immunoglobulin-like (Big) domains, has been proved to be involved in the interaction of pathogenic Leptospira with mammalian host. In this study, the solution structure of the fourth Big domain of LigA (LigA4 Big domain) from Leptospira interrogans was solved by nuclear magnetic resonance (NMR). The structure of LigA4 Big domain displays a similar bacterial immunoglobulin-like fold compared with other Big domains, implying some common structural aspects of Big domain family. On the other hand, it displays some structural characteristics significantly different from classic Ig-like domain. Furthermore, Stains-all assay and NMR chemical shift perturbation revealed the Ca(2+) binding property of LigA4 Big domain.

PubMed: 26449456
DOI: 10.1016/j.bbrc.2015.09.170

Experimental method

SOLUTION NMR