2N0T
Structural ensemble of the enzyme cyclophilin reveals an orchestrated mode of action at atomic resolution
Summary for 2N0T
| Entry DOI | 10.2210/pdb2n0t/pdb |
| Related | 2MZU |
| Descriptor | Peptidyl-prolyl cis-trans isomerase A (1 entity in total) |
| Functional Keywords | isomerase |
| Biological source | Homo sapiens (human) |
| Cellular location | Cytoplasm : P62937 |
| Total number of polymer chains | 1 |
| Total formula weight | 18036.50 |
| Authors | Chi, C.N.,Voegeli, B.,Bibow, S.,Strotz, D.,Orts, J.,Guntert, P.,Riek, R. (deposition date: 2015-03-13, release date: 2015-08-26, Last modification date: 2024-05-15) |
| Primary citation | Chi, C.N.,Vogeli, B.,Bibow, S.,Strotz, D.,Orts, J.,Guntert, P.,Riek, R. A Structural Ensemble for the Enzyme Cyclophilin Reveals an Orchestrated Mode of Action at Atomic Resolution. Angew.Chem.Int.Ed.Engl., 54:11657-11661, 2015 Cited by PubMed Abstract: For enzyme activity, an exact structural and motional orchestration of the active site and its surroundings is believed to be key. In order to reveal such possible phenomena at atomic resolution on the basis of experimental evidence, an experimental restraint driven two-state ensemble of the prototypical enzyme cyclophilin was determined by using a recently introduced exact NOE approach. The ensemble description reveals the presence of an open and a closed state of cyclophilin, which is indicative of large-scale correlated motion. In the open state, the catalytic site is preorganized for catalysis, thus suggesting the mechanism of action to be conformational sampling, while the ligand-binding loop appears to act through an induced fit mechanism. This finding is supported by affinity measurements of a cyclophilin designed to be more open. Overall, more than 60-70 % of the side-chain conformations of cyclophilin appear to be correlated. PubMed: 26265096DOI: 10.1002/anie.201503698 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
