2MOR

A tensor-free method for the structural and dynamical refinement of proteins using residual dipolar couplings

Summary for 2MOR

• Entry DOI: 10.2210/pdb2mor/pdb
• Related: 1D3Z
• NMR Information: BMRB: 6457
• Descriptor: Ubiquitin (1 entity in total)
• Functional Keywords: ubiquitin, signaling protein
• Biological source: Homo sapiens (human)
• Cellular location: Ubiquitin: Cytoplasm : P0CG48
• Total number of polymer chains: 1
• Total formula weight: 8576.83

Authors

Camilloni, C.,Vendruscolo, M. (deposition date: 2014-04-29, release date: 2014-06-25, Last modification date: 2024-05-01)

Primary citation

Camilloni, C.,Vendruscolo, M.
A Tensor-Free Method for the Structural and Dynamical Refinement of Proteins using Residual Dipolar Couplings.
J.Phys.Chem.B, 119:653-661, 2015

PubMed Abstract: Residual dipolar couplings (RDCs) are parameters measured in nuclear magnetic resonance spectroscopy that can provide exquisitely detailed information about the structure and dynamics of biological macromolecules. We describe here a method of using RDCs for the structural and dynamical refinement of proteins that is based on the observation that the RDC between two atomic nuclei depends directly on the angle ϑ between the internuclear vector and the external magnetic field. For every pair of nuclei for which an RDC is available experimentally, we introduce a structural restraint to minimize the deviation from the value of the angle ϑ derived from the measured RDC and that calculated in the refinement protocol. As each restraint involves only the calculation of the angle ϑ of the corresponding internuclear vector, the method does not require the definition of an overall alignment tensor to describe the preferred orientation of the protein with respect to the alignment medium. Application to the case of ubiquitin demonstrates that this method enables an accurate refinement of the structure and dynamics of this protein to be obtained.

PubMed: 24824082
DOI: 10.1021/jp5021824

Experimental method

SOLUTION NMR