2MFP
Solution structure of the circular g-domain analog from the wheat metallothionein Ec-1
Summary for 2MFP
| Entry DOI | 10.2210/pdb2mfp/pdb |
| Related | 2L61 2L62 |
| NMR Information | BMRB: 19557 |
| Descriptor | EC protein I/II, CADMIUM ION (2 entities in total) |
| Functional Keywords | metallothionein, metal-thiolate cluster, backbone cyclized protein, metal binding protein |
| Biological source | Triticum aestivum (Canadian hard winter wheat,common wheat,wheat) |
| Total number of polymer chains | 1 |
| Total formula weight | 3016.96 |
| Authors | Tarasava, K.,Johannsen, S.,Freisinger, E. (deposition date: 2013-10-14, release date: 2013-11-27, Last modification date: 2024-11-06) |
| Primary citation | Tarasava, K.,Johannsen, S.,Freisinger, E. Solution Structure of the Circular gamma-Domain Analog from the Wheat Metallothionein Ec-1. Molecules, 18:14414-14429, 2013 Cited by PubMed Abstract: The first cyclic analog of a metallothionein (MT) was prepared and analyzed by UV and (magnetic) circular dichroism spectroscopy, ESI-MS as well as NMR spectroscopy. Results reveal that the evaluated cyclic γ-E(c)-1 domain of the wheat MT E(c)-1 retains its ability to coordinate two Zn(II) or Cd(II) ions and adopts a three-dimensional structure that is highly similar to the one of the linear wild-type form. However, the reduced flexibility of the protein backbone facilitates structure solution significantly and results in a certain stabilization of metal binding to the protein. PubMed: 24284492DOI: 10.3390/molecules181114414 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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