2MA9

HIV-1 Vif SOCS-box and Elongin BC solution structure

Summary for 2MA9

• Entry DOI: 10.2210/pdb2ma9/pdb
• Related: 3DCG
• NMR Information: BMRB: 19333
• Descriptor: Virion infectivity factor, Transcription elongation factor B polypeptide 2, Transcription elongation factor B polypeptide 1 (3 entities in total)
• Functional Keywords: hiv-1 vif socs-box, elongin bc, viral protein-protein binding complex, viral protein/protein binding
• Biological source: Human immunodeficiency virus type 1 (HIV-1); More
• Cellular location: Host cytoplasm: P12504; Nucleus : Q15370 Q15369
• Total number of polymer chains: 3
• Total formula weight: 27483.37

Authors

Lu, Z.,Bergeron, J.R.,Atkinson, R.A.,Schaller, T.,Veselkov, D.A.,Oregioni, A.,Yang, Y.,Matthews, S.J.,Malim, M.H.,Sanderson, M.R. (deposition date: 2013-07-01, release date: 2013-12-11, Last modification date: 2024-05-01)

Primary citation

Lu, Z.,Bergeron, J.R.,Atkinson, R.A.,Schaller, T.,Veselkov, D.A.,Oregioni, A.,Yang, Y.,Matthews, S.J.,Malim, M.H.,Sanderson, M.R.
Insight into the HIV-1 Vif SOCS-box-ElonginBC interaction.
OPEN BIOLOGY, 3:130100-130100, 2013

PubMed Abstract: The HIV-1 viral infectivity factor (Vif) neutralizes cell-encoded antiviral APOBEC3 proteins by recruiting a cellular ElonginB (EloB)/ElonginC (EloC)/Cullin5-containing ubiquitin ligase complex, resulting in APOBEC3 ubiquitination and proteolysis. The suppressors-of-cytokine-signalling-like domain (SOCS-box) of HIV-1 Vif is essential for E3 ligase engagement, and contains a BC box as well as an unusual proline-rich motif. Here, we report the NMR solution structure of the Vif SOCS-ElonginBC (EloBC) complex. In contrast to SOCS-boxes described in other proteins, the HIV-1 Vif SOCS-box contains only one α-helical domain followed by a β-sheet fold. The SOCS-box of Vif binds primarily to EloC by hydrophobic interactions. The functionally essential proline-rich motif mediates a direct but weak interaction with residues 101-104 of EloB, inducing a conformational change from an unstructured state to a structured state. The structure of the complex and biophysical studies provide detailed insight into the function of Vif's proline-rich motif and reveal novel dynamic information on the Vif-EloBC interaction.

PubMed: 24225024
DOI: 10.1098/rsob.130100

Experimental method

SOLUTION NMR