2LS6

Solution NMR Structure of a Non-canonical galactose-binding CBM32 from Clostridium perfringens

Summary for 2LS6

• Entry DOI: 10.2210/pdb2ls6/pdb
• NMR Information: BMRB: 17694
• Descriptor: Hyaluronoglucosaminidase (1 entity in total)
• Functional Keywords: glycoside hydrolases, carbohyrate-binding modules, galactose, hydrolase
• Biological source: Clostridium perfringens
• Total number of polymer chains: 1
• Total formula weight: 18352.35

Authors

Grondin, J.M.,Chitayat, S.,Ficko-Blean, E.,Boraston, A.B.,Smith, S.P. (deposition date: 2012-04-20, release date: 2013-05-01, Last modification date: 2024-05-01)

Primary citation

Grondin, J.M.,Chitayat, S.,Ficko-Blean, E.,Houliston, S.,Arrowsmith, C.H.,Boraston, A.B.,Smith, S.P.
An unusual mode of galactose recognition by a family 32 carbohydrate-binding module.
J.Mol.Biol., 426:869-880, 2014

PubMed Abstract: Carbohydrate-binding modules (CBMs) are ancillary modules commonly associated with carbohydrate-active enzymes (CAZymes) that function to mediate the adherence of the parent enzyme to its carbohydrate substrates. CBM family 32 (CBM32) is one of the most diverse CBM families, whose members are commonly found in bacterial CAZymes that modify eukaryotic glycans. One such example is the putative μ-toxin, CpGH84A, of the family 84 glycoside hydrolases, which comprises an N-terminal putative β-N-acetylglucosaminidase catalytic module and four tandem CBM32s. Here, we report a unique mode of galactose recognition by the first CBM32, CBM32-1 from CpGH84A. Solution NMR-based analyses of CpGH84A CBM32-1 indicate a divergent subset of residues, located in ordered loops at the apex of the CBM, conferring specificity for the galacto-configured sugars galactose, GalNAc, and LacNAc that differs from those of the canonical galactose-binding CBM32s. This study showcases the impressive variability in ligand binding by this CBM family and offers insight into the growing role of these modules in the interaction of CAZymes with eukaryotic glycans.

PubMed: 24326248
DOI: 10.1016/j.jmb.2013.11.029

Experimental method

SOLUTION NMR