2LKS

Ff11-60

Summary for 2LKS

• Entry DOI: 10.2210/pdb2lks/pdb
• Related: 1UZC 2KZG
• NMR Information: BMRB: 18010
• Descriptor: Pre-mRNA-processing factor 40 homolog A (1 entity in total)
• Functional Keywords: protein binding
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus speckle (By similarity): O75400
• Total number of polymer chains: 1
• Total formula weight: 5775.67

Authors

Barette, J.,Velyvis, A.,Religa, T.L.,Korzhnev, D.M.,Kay, L.E. (deposition date: 2011-10-19, release date: 2012-01-11, Last modification date: 2024-05-15)

Primary citation

Barette, J.,Velyvis, A.,Religa, T.L.,Korzhnev, D.M.,Kay, L.E.
Cross-Validation of the Structure of a Transiently Formed and Low Populated FF Domain Folding Intermediate Determined by Relaxation Dispersion NMR and CS-Rosetta.
J.Phys.Chem.B, 116:6637-6644, 2012

PubMed Abstract: We have recently reported the atomic resolution structure of a low populated and transiently formed on-pathway folding intermediate of the FF domain from human HYPA/FBP11 [Korzhnev, D. M.; Religa, T. L.; Banachewicz, W.; Fersht, A. R.; Kay, L.E. Science 2011, 329, 1312-1316]. The structure was determined on the basis of backbone chemical shift and bond vector orientation restraints of the invisible intermediate state measured using relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy that were subsequently input into the database structure determination program, CS-Rosetta. As a cross-validation of the structure so produced, we present here the solution structure of a mimic of the folding intermediate that is highly populated in solution, obtained from the wild-type domain by mutagenesis that destabilizes the native state. The relaxation dispersion/CS-Rosetta structures of the intermediate are within 2 Å of those of the mimic, with the nonnative interactions in the intermediate also observed in the mimic. This strongly confirms the structure of the FF domain folding intermediate, in particular, and validates the use of relaxation dispersion derived restraints in structural studies of invisible excited states, in general.

PubMed: 22148426
DOI: 10.1021/jp209974f

Experimental method

SOLUTION NMR