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2LJ7

3D solution structure of plant defensin Lc-def

Summary for 2LJ7
Entry DOI10.2210/pdb2lj7/pdb
NMR InformationBMRB: 17923
DescriptorDefensin Lc-def (1 entity in total)
Functional Keywordsantimicrobial protein
Biological sourceLens culinaris subsp. culinaris (Cultivated lentil)
Cellular locationSecreted : B3F051
Total number of polymer chains1
Total formula weight5460.24
Authors
Shenkarev, Z.,Mineev, K.,Gizatullina, A. (deposition date: 2011-09-07, release date: 2012-09-12, Last modification date: 2024-11-20)
Primary citationShenkarev, Z.O.,Gizatullina, A.K.,Finkina, E.I.,Alekseeva, E.A.,Balandin, S.V.,Mineev, K.S.,Arseniev, A.S.,Ovchinnikova, T.V.
Heterologous expression and solution structure of defensin from lentil Lens culinaris.
Biochem.Biophys.Res.Commun., 451:252-257, 2014
Cited by
PubMed Abstract: A new defensin Lc-def, isolated from germinated seeds of the lentil Lens culinaris, has molecular mass 5440.4Da and consists of 47 amino acid residues. Lc-def and its (15)N-labeled analog were overexpressed in Escherichia coli. Antimicrobial activity of the recombinant protein was examined, and its spatial structure, dynamics, and interaction with lipid vesicles were studied by NMR spectroscopy. It was shown that Lc-def is active against fungi, but does not inhibit the growth of Gram-positive and Gram-negative bacteria. The peptide is monomeric in aqueous solution and contains one α-helix and triple-stranded β-sheet, which form cysteine-stabilized αβ motif (CSαβ) previously found in other plant defensins. The sterically neighboring loop1 and loop3 protrude from the defensin core and demonstrate significant mobility on the μs-ms timescale. Lc-def does not bind to the zwitterionic lipid (POPC) vesicles but interacts with the partially anionic (POPC/DOPG, 7:3) membranes under low-salt conditions. The Lc-def antifungal activity might be mediated through electrostatic interaction with anionic lipid components of fungal membranes.
PubMed: 25086358
DOI: 10.1016/j.bbrc.2014.07.104
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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