2LI6

1H, 13C, and 15N Chemical Shift Assignments for yeast protein

Summary for 2LI6

• Entry DOI: 10.2210/pdb2li6/pdb
• NMR Information: BMRB: 17880
• Descriptor: SWI/SNF chromatin-remodeling complex subunit SWI1 (1 entity in total)
• Functional Keywords: ligand binding, dna binding protein
• Biological source: Saccharomyces cerevisiae (yeast)
• Cellular location: Nucleus: P09547
• Total number of polymer chains: 1
• Total formula weight: 13818.96

Authors

Wang, T.,Zhang, J.,Tu, X. (deposition date: 2011-08-24, release date: 2012-04-25, Last modification date: 2024-05-15)

Primary citation

Wang, T.,Zhang, J.,Zhang, X.,Tu, X.
Solution structure of SWI1 ARID domain from Saccharomyces cerevisiae and its non-specific binding to DNA
Proteins, 2012

PubMed Abstract: SWI1 is a subunit of the SWI/SNF complex involved in chromatin remodeling. It contains an AT-rich interaction domain (ARID) which has the potential DNA binding activity. In this study, we determined the solution structure of the SWI1 ARID domain from Saccharomyces cerevisiae by nuclear magnetic resonance spectroscopy. Yeast SWI1 ARID domain is composed of seven alpha helices, six of which are conserved among the ARID family. In addition, the DNA-binding activity of the SWI1 ARID domain was confirmed by chemical shift perturbation assay. Similar to its human homolog, the yeast SWI1 ARID domain binds DNA nonspecifically.

PubMed: 22488857
DOI: 10.1002/prot.24091

Experimental method

SOLUTION NMR