Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

2L9U

Spatial structure of dimeric ErbB3 transmembrane domain

Summary for 2L9U
Entry DOI10.2210/pdb2l9u/pdb
NMR InformationBMRB: 17488
DescriptorReceptor tyrosine-protein kinase erbB-3 (1 entity in total)
Functional Keywordstransmenbrane dimer, membrane protein, erbb, egfr
Biological sourceHomo sapiens (human)
Cellular locationIsoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted: P21860
Total number of polymer chains2
Total formula weight9407.32
Authors
Mineev, K.S.,Arseniev, A.S. (deposition date: 2011-02-24, release date: 2011-05-18, Last modification date: 2024-05-15)
Primary citationMineev, K.S.,Khabibullina, N.F.,Lyukmanova, E.N.,Dolgikh, D.A.,Kirpichnikov, M.P.,Arseniev, A.S.
Spatial structure and dimer--monomer equilibrium of the ErbB3 transmembrane domain in DPC micelles.
Biochim. Biophys. Acta, 1808:2081-2088, 2011
Cited by
PubMed Abstract: In present work the interaction of two TM α-helices of the ErbB3 receptor tyrosine kinase from the ErbB or HER family (residues 639-670) was studied by means of NMR spectroscopy in a membrane-mimicking environment provided by the DPC micelles. The ErbB3 TM segment appeared to form a parallel symmetric dimer in a left-handed orientation. The interaction between TM spans is accomplished via the non-standard motif and is supported by apolar contacts of bulky side chains and by stacking of aromatic rings together with π-cation interactions of Phe and Arg side chains. The investigation of the dimer--monomer equilibrium revealed thermodynamic properties of the assembly and the presence of two distinct regimes of the dimerization at low and at high peptide/detergent ratio. It was found that the detergent in case of ErbB3 behaves not as an ideal solvent, thus affecting the dimer--monomer equilibrium. Such behavior may account for the problems occurring with the refolding and stability of multispan helical membrane proteins in detergent solutions. The example of ErbB3 allows us to conclude that the thermodynamic parameters of dimerization, measured in micelles for two different helical pairs, cannot be compared without the investigation of their dependence on detergent concentration.
PubMed: 21575594
DOI: 10.1016/j.bbamem.2011.04.017
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

227344

PDB entries from 2024-11-13

PDB statisticsPDBj update infoContact PDBjnumon