2L8X
Spatial structure of antimicrobial peptide Arenicin-2 dimer in DPC micelles
Summary for 2L8X
| Entry DOI | 10.2210/pdb2l8x/pdb |
| NMR Information | BMRB: 17430 |
| Descriptor | Arenicin-2 (1 entity in total) |
| Functional Keywords | arenicin, oligomerization, beta-hairpin, pore forming peptide, antimicrobial protein |
| Biological source | Arenicola marina (Lugworm) |
| Total number of polymer chains | 2 |
| Total formula weight | 5560.75 |
| Authors | Shenkarev, Z.O.,Trunov, K.I.,Paramonov, A.S.,Arseniev, A.S. (deposition date: 2011-01-27, release date: 2011-12-14, Last modification date: 2024-10-09) |
| Primary citation | Shenkarev, Z.O.,Balandin, S.V.,Trunov, K.I.,Paramonov, A.S.,Sukhanov, S.V.,Barsukov, L.I.,Arseniev, A.S.,Ovchinnikova, T.V. Molecular mechanism of action of beta-hairpin antimicrobial peptide arenicin: oligomeric structure in dodecylphosphocholine micelles and pore formation in planar lipid bilayers Biochemistry, 50:6255-6265, 2011 Cited by PubMed Abstract: The membrane-active, cationic, β-hairpin peptide, arenicin, isolated from marine polychaeta Arenicola marina exhibits a broad spectrum of antimicrobial activity. The peptide in aqueous solution adopts the significantly twisted β-hairpin conformation without pronounced amphipathicity. To assess the mechanism of arenicin action, the spatial structure and backbone dynamics of the peptide in membrane-mimicking media and its pore-forming activity in planar lipid bilayers were studied. The spatial structure of the asymmetric arenicin dimer stabilized by parallel association of N-terminal strands of two β-hairpins was determined using triple-resonance nuclear magnetic resonance (NMR) spectroscopy in dodecylphosphocholine (DPC) micelles. Interaction of arenicin with micelles and its oligomerization significantly decreased the right-handed twist of the β-hairpin, increased its amphipathicity, and led to stabilization of the peptide backbone on a picosecond to nanosecond time scale. Relaxation enhancement induced by water-soluble (Mn(2+)) and lipid-soluble (16-doxylstearate) paramagnetic probes pointed to the dimer transmembrane arrangement. Qualitative NMR and circular dichroism study of arenicin-2 in mixed DPC/1,2-dioleoyl-sn-glycero-3-phosphoglycerol bicelles, sodium dodecyl sulfate micelles, and lipid vesicles confirmed that a similar dimeric assembly of the peptide was retained in membrane-mimicking systems containing negatively charged lipids and detergents. Arenicin-induced conductance was dependent on the lipid composition of the membrane. Arenicin low-conductivity pores were detected in the phosphatidylethanolamine-containing lipid mixture, whereas the high-conductivity pores were observed in an exclusively anionic lipid system. The measured conductivity levels agreed with the model in which arenicin antimicrobial activity was mediated by the formation of toroidal pores assembled of two, three, or four β-structural peptide dimers and lipid molecules. The structural transitions involved in arenicin membrane-disruptive action are discussed. PubMed: 21627330DOI: 10.1021/bi200746t PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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