2KVE

C-terminal domain of mesencephalic astrocyte-derived neurotrophic factor (MANF)

Summary for 2KVE

• Entry DOI: 10.2210/pdb2kve/pdb
• Related: 2KVD
• NMR Information: BMRB: 16776
• Descriptor: Mesencephalic astrocyte-derived neurotrophic factor (1 entity in total)
• Functional Keywords: disulfide bond, glycoprotein, growth factor, secreted, sialic acid, unfolded protein response, hormone
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P55145
• Total number of polymer chains: 1
• Total formula weight: 7476.78

Authors

Hellman, M.H.,Saarma, M.,Permi, P. (deposition date: 2010-03-12, release date: 2010-11-03, Last modification date: 2024-10-16)

Primary citation

Hellman, M.H.,Arumae, U.,Yu, L.-Y.,Lindholm, P.,Peranen, J.,Saarma, M.,Permi, P.
Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) Has a Unique Mechanism to Rescue Apoptotic Neurons
J.Biol.Chem., 286:2675-2680, 2011

PubMed Abstract: Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects neurons and repairs the Parkinson disease-like symptoms in a rat 6-hydroxydopamine model. We show a three-dimensional solution structure of human MANF that differs drastically from other neurotrophic factors. Remarkably, the C-terminal domain of MANF (C-MANF) is homologous to the SAP domain of Ku70, a well known inhibitor of proapoptotic Bax (Bcl-2-associated X protein). Cellular studies confirm that MANF and C-MANF protect neurons intracellularly as efficiently as Ku70.

PubMed: 21047780
DOI: 10.1074/jbc.M110.146738

Experimental method

SOLUTION NMR