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2JOW

Differences in the electrostatic surfaces of the type III secretion needle proteins

Summary for 2JOW
Entry DOI10.2210/pdb2jow/pdb
NMR InformationBMRB: 15206
DescriptorProtein prgI (1 entity in total)
Functional Keywordsneedle protein, transport protein
Biological sourceSalmonella typhimurium
Total number of polymer chains1
Total formula weight9276.26
Authors
Wang, Y.,Ouellette, A.N.,Egan, C.W.,De Guzman, R.N. (deposition date: 2007-04-06, release date: 2007-07-24, Last modification date: 2024-05-08)
Primary citationWang, Y.,Ouellette, A.N.,Egan, C.W.,Rathinavelan, T.,Im, W.,De Guzman, R.N.
Differences in the Electrostatic Surfaces of the Type III Secretion Needle Proteins PrgI, BsaL, and MxiH
J.Mol.Biol., 371:1304-1314, 2007
Cited by
PubMed Abstract: Gram-negative bacteria use a needle-like protein assembly, the type III secretion apparatus, to inject virulence factors into target cells to initiate human disease. The needle is formed by the polymerization of approximately 120 copies of a small acidic protein that is conserved among diverse pathogens. We previously reported the structure of the BsaL needle monomer from Burkholderia pseudomallei by nuclear magnetic resonance (NMR) spectroscopy and others have determined the crystal structure of the Shigella flexneri MxiH needle. Here, we report the NMR structure of the PrgI needle protein of Salmonella typhimurium, a human pathogen associated with food poisoning. PrgI, BsaL, and MxiH form similar two helix bundles, however, the electrostatic surfaces of PrgI differ radically from those of BsaL or MxiH. In BsaL and MxiH, a large negative area is on a face formed by the helix alpha1-alpha2 interface. In PrgI, the major negatively charged surface is not on the "face" but instead is on the "side" of the two-helix bundle, and only residues from helix alpha1 contribute to this negative region. Despite being highly acidic proteins, these molecules contain large basic regions, suggesting that electrostatic contacts are important in needle assembly. Our results also suggest that needle-packing interactions may be different among these bacteria and provide the structural basis for why PrgI and MxiH, despite 63% sequence identity, are not interchangeable in S. typhimurium and S. flexneri.
PubMed: 17617421
DOI: 10.1016/j.jmb.2007.06.034
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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