2HO7
Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme bound to glucose-6-phosphate
Summary for 2HO7
| Entry DOI | 10.2210/pdb2ho7/pdb |
| Related | 2HO6 |
| Descriptor | glmS ribozyme substrate RNA, glmS ribozyme RNA, 6-O-phosphono-alpha-D-glucopyranose, ... (5 entities in total) |
| Functional Keywords | rna; ribozyme; pseudoknot; helix, rna |
| Total number of polymer chains | 2 |
| Total formula weight | 49564.59 |
| Authors | Klein, D.J.,Ferre-D'Amare, A.R. (deposition date: 2006-07-13, release date: 2006-09-26, Last modification date: 2024-02-14) |
| Primary citation | Klein, D.J.,Ferre-D'Amare, A.R. Structural basis of glmS ribozyme activation by glucosamine-6-phosphate Science, 313:1752-1756, 2006 Cited by PubMed Abstract: The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis. PubMed: 16990543DOI: 10.1126/science.1129666 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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