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2DU0

Crystal structure of basic winged bean lectin in complex with Alpha-D-galactose

Summary for 2DU0
Entry DOI10.2210/pdb2du0/pdb
Related1wbf 1wbl 2DTW 2DU1 2DUY 2d3s
DescriptorBasic agglutinin, alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-L-fucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
Functional Keywordslegume lectin, glycosylated protein, agglutinin, sugar binding protein
Biological sourcePsophocarpus tetragonolobus (winged bean)
Total number of polymer chains4
Total formula weight109865.70
Authors
Kulkarni, K.A.,Katiyar, S.,Surolia, A.,Vijayan, M.,Suguna, K. (deposition date: 2006-07-19, release date: 2006-11-07, Last modification date: 2024-10-23)
Primary citationKulkarni, K.A.,Katiyar, S.,Surolia, A.,Vijayan, M.,Suguna, K.
Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc
ACTA CRYSTALLOGR.,SECT.D, 62:1319-1324, 2006
Cited by
PubMed Abstract: The crystal structure of the complexes of basic winged-bean lectin with galactose, 2-methoxygalactose, N-acetylgalactosamine and methyl-alpha-N-acetylgalactosamine have been determined. Lectin-sugar interactions involve four hydrogen bonds and a stacking interaction in all of the complexes. In addition, an N-H...O hydrogen bond involving the hydroxyl group at C2 exists in the galactose and 2-methoxygalactose complexes. An additional hydrophobic interaction involving the methyl group in the latter leads to the higher affinity of the methyl derivative. In the lectin-N-acetylgalactosamine complex the N-H...O hydrogen bond is lost, but a compensatory hydrogen bond is formed involving the O atom of the acetamido group. In addition, the CH(3) moiety of the acetamido group is involved in hydrophobic interactions. Consequently, the 2-methyl and acetamido derivatives of galactose have nearly the same affinity for the lectin. The methyl group alpha-linked to the galactose takes part in additional hydrophobic interactions. Therefore, methyl-alpha-N-acetylgalactosamine has a higher affinity than N-acetylgalactosamine for the lectin. The structures of basic winged-bean lectin-sugar complexes provide a framework for examining the relative affinity of galactose and galactosamine for the lectins that bind to them. The complexes also lead to a structural explanation for the blood-group specificity of basic winged-bean lectin.
PubMed: 17057334
DOI: 10.1107/S0907444906028198
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.7 Å)
Structure validation

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